FIGURE 1.

Structural features of Eg5·AMPPNP. a, F_o − F_c simulated annealing omit density map contoured at 3σ surrounds the AMPPNP, Mg²⁺, W1, and W2. b, highlighted in the Eg5·AMPPNP complex are L9 (yellow) containing switch I, L11 (green) containing switch II, the P-loop (pink), and the L5-loop (red), which is involved in binding allosteric inhibitors. AMPPNP and Mg²⁺ (dark sphere) are also shown. c, comparison of α6, α4, and the neck linker of Eg5·AMPPNP (red), Eg5·ADP (cyan), and Kif1A·AMPPNP (yellow) after superimposing the structures (see “Experimental Procedures”).