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. 2009 Dec 18;285(8):5878–5887. doi: 10.1074/jbc.M109.033761

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Rottlerin and SP600125 decreased the migration rate of locomoting neurons. A–E, time-lapse observation of locomoting neurons in cultured cortical tissues with or without rottlerin (1 or 5 μm), safingol, or SP600125. Cortical slices were cultured without any inhibitors for the first 4 h as an internal control (white arrowheads). Subsequently, we added the indicated inhibitors into cultured media (red arrows), and continued to observe the same locomoting neurons for an additional 4 h (red arrowheads). Green, EGFP; red, DsRed with NLS; scale bar, 20 μm.