FIGURE 5.

mc-PAF elicits an increase in intracellular Ca²⁺ through the mutant HA-PAFRs sorted to the cell surface by Y-24180. A, the mc-PAF-elicited intracellular Ca²⁺ increase in HeLa cells transfected with WT HA-hPAFR, D63A, P247A, or empty vector with Y-24180 (1 μm) treatment was measured with a FlexStation fluorometer. Relative fluorescence units (RFU, Max − Min) are indicated. Data are represented as mean ± S.E. (n = 3). B, a representative result from the flow cytometric analysis is shown. HeLa cells were transiently transfected with WT HA-hPAFR, D63A, P247A, or empty vector, then treated with Y-24180 (1 μm).