TABLE 1.
Germination times of gsmA and sdmA mutant spores under osmotic stress
| Spore type | Germination time± SD (h)a |
||
|---|---|---|---|
| No addition | 0.2 M NaCl | 0.2 M sucrose | |
| Wild type | 56.3 ± 7.5 | 86.6 ± 8.1 | 83.3 ± 9.6 |
| gsmA | 54.6 ± 12.5 | 107.6 ± 7.4 | 109.9 ± 18.1 |
| sdmA | 64.6 ± 1.9 | 119.6 ± 14.0 | 121.6 ± 13.6 |
a
Germination time is the time at which about 50% of spores had germinated. Spores were harvested from 6- to 8-day-old fruiting bodies on CF plates, sonicated for 2 min, and treated with heat (60°C for 15 min). The spores were inoculated to 2 × 107 spores/ml in CYE medium containing 0.2 M NaCl or sucrose and incubated at 30°C with continuous shaking. The ungerminated spores in each culture were counted using a hemocytometer. Experiments were repeated three times.