FIG. 3.

Venn diagram of S. mutans genes that are subject to metalloregulation. The Venn diagram reveals the numbers of S. mutans genes that were identified in one or more of our genome-wide analyses. Only genes that were significantly manganese and/or SloR regulated (P < 0.05) and/or preceded by a predicted SRE are represented. We selected the S. mutans genes that reside at the intersects of all three analyses (shaded region) for further investigation because (i) many of them encode S. mutans virulence attributes (e.g., pdh, tpx, citZ, bta, and glgBCDA), (ii) they are manganese responsive, (iii) they are expressed differently in the wild-type UA159 strain and the GMS584 SloR-deficient mutant and hence are SloR-regulated, and (iv) they are preceded by a predicted SRE. For the PROMSCAN and MAST analyses of the SRE data set, we applied a PERL script to remove duplications.