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. 2009 Dec 18;192(5):1221–1230. doi: 10.1128/JB.01312-09

FIG. 1.

FIG. 1.

Localization of the DNA region responsible for the segregational stability of pBET131. Plasmid stability was determined as described in Materials and Methods and is shown as percent plasmid loss per generation. (A) Restriction map of pBET131. The DNA region between the BamHI and HindIII sites was derived from pLS32 (46). The open arrows show the open reading frames on pBET131. Abbreviations: Ba, BamHI; Bg, BglII; Cl, ClaI; Cs, Csp45I; Hi, HindIII; Nh, NheI; Nr, NruI; Sa, SalI; Sc, SacI; Sn, SnaBI; Xh, XhoI. (B) Derivatives of pBET131 carrying modifications in the upstream region of alfA. The rectangle and the dotted lines show the Pspac promoter and the deleted region between positions 3033 and 3060, which contains part of the promoter for the alf operon (Fig. 2B), respectively. The three arrows depict the tandem repeat sequence located between positions 3000 and 3031. (C) Derivatives of pBET131 carrying synthetic 55-nucleotide sequences between the BamHI and SnaBI (position 3032) sites of pBET131. The arrows and ×s show the tandem repeats and nucleotide changes, respectively.