Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Derivatives of pDG148 carrying alfA and alfB downstream of the IPTG-inducible Pspac promoter. The solid bars depict the DNA regions carried in the plasmids. The filled triangles show the SacI sites that were blunt ended with T4 DNA polymerase and ligated. For the abbreviations of the restriction enzymes, see the legend to Fig. 1. oriB, replication origin for B. subtilis; oriE, replication origin for E. coli.
