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Repression of alfA expression by AlfB. The cells of strain LA213 (amyE::alfA-lacZ) carrying the plasmids were cultured in the presence of Cm, Nm, and IPTG, and the β-galactosidase activities were determined as described in Materials and Methods. The structures of the plasmids are shown in Fig. 3. ○, pDG148; •, pENSA51; □, pEXSA564.
