TABLE 1.
Strainsa
| Strain | Genotype/remarks | Source or reference |
|---|---|---|
| A. actinomycetemcomitans | ||
| VT1169 | Wild type, a Rifr Nalr derivative of SUNY465, serotype b | 24 |
| VT1169 (pKM2/emaA) | VT1169 transformed with plasmid pKM2/emaA (EmaA) | This study |
| emaA mutant (emaA::Sp) | Spectinomycin adenyltransferase gene (aad9) inserted into emaA | 24 |
| rmlC mutant (rmlC::pLOF/Sp) | Transposon pLOF/Sp inserted into rmlC (TDP-4-keto-6-deoxy-d-glucose 3,5-epimerase) | This study |
| rmlC mutant/rmlC | rmlC mutant complemented with plasmid pKM2/ltxP/rmlC | This study |
| wzt mutant (wzt::pLOF/Sp) | Transposon pLOF/Sp inserted into wzt (ATP binding cassette transporter) | This study |
| waaL mutant (waaL::pKM221) | Plasmid pKM221 inserted into waaL (O-antigen ligase) | This study |
| waaL mutant/waaL | waaL mutant complemented with plasmid pKM2/ltxP/waaL | This study |
| E. coli | ||
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lazZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(are, leu)7697 galU galK λ−rpsL nupG tonA | Invitrogen, Carlsbad, CA |
| DH5α (λ pir) | endA1 hadR17(r− m+) supE44 thi-1 recA gyrA1(Nalr) relA1 Δ(lacIZYA-argF)U169 deoR (φ80dlacΔ(lacZ)M15) λ pir | 25 |
| SM10 (λ pir) | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km pir R6K | 25 |
a
Rif, rifampin; Nal, nalidixic acid; Sp, spectinomycin.