TABLE 2.
Plasmidsa
| Plasmid | Description | Reference |
|---|---|---|
| pVT1461 | A derivative of pGP704, containing the spectinomycin adenyltransferase gene (aad9), Spr | 25 |
| pKM221 | pVT1461 containing 5-545 bp of waaL, Spr | This study |
| pLOF/Sp | Tn10-based transposon vector; Apr Spr | 24 |
| pKM2 | pPK1 containing chloramphenicol acetyltransferase, Cmr | 10 |
| pKM2/emaA | pKM2 containing ∼500-bp upstream sequence of emaA (the putative promoter region) + emaA sequence, Cmr | This study |
| pKM2/ltxP | pKM2 containing ∼500-bp ltx promoter, Cmr | This study |
| pKM2/ltxP/rmlC | pKM2 containing ∼500-bp ltx promoter + rmlC sequence, Cmr | This study |
| pKM2/ltxP/waaL | pKM2 containing ∼500-bp ltx promoter + waaL sequence, Cmr | This study |
a
Ap, ampicillin; Cm, chloramphenicol; Sp, spectinomycin.