TABLE 2.
Important genes of S. mutans differentially expressed in the ΔclpP strain (IBS512)
| Gene name | Description and/or putative function | Fold change relative to WTa |
|---|---|---|
| Stress related | ||
| SMU.22 | Putative peptidoglycan hydrolase; GbpB | 1.5 |
| SMU.81 | Heat shock protein HSP-70 cofactor; GrpE | 2.1 |
| SMU.82 | Molecular chaperone; DnaK | 1.9 |
| SMU.83 | Heat shock protein HSP-40; DnaJ | 2.1 |
| SMU.140 | Glutathione reductase | −3.2 |
| SMU.561 | Nudix hydrolase (DNA repair) | 1.6 |
| SMU.562 | ATP-dependent protease; ClpE | 1.9 |
| SMU.949 | ATP-dependent protease; ClpX | 1.5 |
| SMU.991 | Putative ribonucleotide reductase | 1.5 |
| SMU.1954 | Chaperonin GroEL | 1.7 |
| SMU.1955 | Cochaperonin GroES | 1.6 |
| SMU.2084 | Transcriptional regulator SpxA | 1.8 |
| SMU.2164 | Serine protease HtrA | 2.0 |
| GIs | ||
| GI-4 | ||
| SMU.100 | Putative sorbose PTS system, IIB | −2.7 |
| SMU.101 | Putative sorbose PTS system, IIC | −1.6 |
| SMU.107 | Hypothetical protein | 1.5 |
| SMU.109 | Putative permease | 1.5 |
| GI-6 (TnSmu1) | ||
| SMU.191 | Putative integrase | 1.9 |
| SMU.193 | Hypothetical protein | 1.8 |
| SMU.194 | Bacteriophage P2 associated | 2.0 |
| SMU.195 | Bacteriophage SPP1 ORF5 related | 1.7 |
| SMU.196 | Putative transfer protein with CHAP | 1.8 |
| domain | ||
| SMU.197 | Hypothetical protein | 2.1 |
| SMU.198 | Putative conjugative transposon protein | 2.6 |
| (helicase) | ||
| SMU.199 | Putative permease/transporter | 3.0 |
| SMU.200 | Hypothetical protein | 3.4 |
| SMU.201 | Putative transposon-related protein | 3.6 |
| SMU.202 | Hypothetical protein | 3.6 |
| SMU204 | Hypothetical protein | 3.2 |
| SMU.205 | Hypothetical protein | 3.0 |
| SMU.206 | Hypothetical protein | 3.6 |
| SMU.207 | XRE family-like protein | 2.9 |
| SMU.208 | ABC transporter | 3.1 |
| SMU.209 | Hypothetical protein | 2.7 |
| SMU.210 | Hypothetical protein | 2.6 |
| SMU.211 | Hypothetical protein | 2.7 |
| SMU.212 | Hypothetical protein | 3.2 |
| SMU.213 | Hypothetical protein | 2.4 |
| SMU.214 | Hypothetical protein | 3.8 |
| SMU.215 | Hypothetical protein | 2.6 |
| SMU.216 | Hypothetical protein | 2.8 |
| SMU.217 | Hypothetical protein | 2.3 |
| SMU.218 | Putative transcriptional regulator | 1.4 |
| SMU.222 | Putative integrase | 1.5 |
| SMU.223 | Hypothetical protein | 1.6 |
| GI-12 (TnSmu2) | ||
| SMU.1339 | Smt operon: BacD; bacitracin synthase | −5.3 |
| SMU.1340 | Smt operon: BacA2; surfactin synthase | −5.8 |
| SMU.1341 | Smt operon: Grs; gramicidin S synthase | −6.7 |
| SMU.1342 | Smt operon: BacA; bacitracin synthase 1 | −6.2 |
| SMU.1343 | Smt operon: PksC; polyketide synthase | −6.5 |
| SMU.1344 | Smt operon: FabD; acyl carrier protein | −0.4 |
| transacylase | ||
| SMU.1345 | Smt operon: ItuA; peptide synthase | −6.9 |
| similar to MycA | ||
| SMU.1346 | Smt operon: BacT; putative thioesterase | −6.0 |
| SMU.1347 | Smt operon: YlbB; ABC transporter | −4.3 |
| permease | ||
| SMU.1348 | Smt operon: PsaA; ABC transporter | −4.4 |
| SMU.1349 | Transcriptional regulator; TetR/AcrR | 1.9 |
| family | ||
| SMU.1365 | ABC transporter permease | −4.1 |
| (= SMU.11347) | ||
| SMU.1366 | ABC transporter (= SMU.1348) | −4.5 |
| CRISPR associated | ||
| SMU.1752 | Hypothetical protein | 1.6 |
| SMU.1753 | CRISPR-associated Cas2 family protein | 1.6 |
| SMU.1754 | CRISPR-associated DNA polymerase | 1.6 |
| SMU.1755 | CRISPR-associated Cas1 family protein | 1.6 |
| SMU.1757 | CRISPR-associated Cas1 family protein | 1.5 |
| SMU.1758 | CRISPR-associated Cas4 family protein | 1.7 |
| SMU.1760 | CRISPR-associated Csd2 family protein | 1.7 |
| SMU.1761 | CRISPR-associated RAMP Csd1 family | 2.1 |
| protein | ||
| SMU.1762 | CRISPR-associated RAMP Csd1 family | 1.8 |
| protein | ||
| SMU.1763 | CRISPR-associated protein Cas5 | 2.0 |
| SMU.1764 | CRISPR-associated helicase Cas3 | 2.7 |
| Bacteriocin related | ||
| SMU.150 | Nonlantibiotic mutacin IV: peptide | −2.3 |
| NlmB | ||
| SMU.151 | Nonlantibiotic mutacin IV: peptide | −2.0 |
| NlmB | ||
| SMU.152 | Putative bacteriocin immunity protein | −2.0 |
| SMU.423 | Putative bacteriocin peptide; BsmC | −1.9 |
| SMU.1897 | ABC transport system CslAB | −1.5 |
| SMU.1898 | −2.0 | |
| SMU1899 | −2.0 | |
| SMU.1900 | −2.2 | |
| SMU.1902 | Putative bacteriocin peptide; BsmK | −2.9 |
| SMU.1905 | Putative bacteriocin peptide; BsmL | −2.1 |
| SMU.1906 | Putative bacteriocin peptide; BsmB | −1.8 |
| SMU.1909 | Putative bacteriocin immunity protein | −1.7 |
| SMU.1910 | Putative bacteriocin immunity protein | −1.5 |
| SMU.1913 | BlpL-like immunity protein | −1.7 |
| SMU.1914 | Mutacin V (NlmC or BsmA) | −1.7 |
a
WT, wild type. P values are given in Table S1 in the supplemental material.