TABLE 4.
Protein spots identified by two-dimensional gel analysis
| Gene name | Protein | No. of peptide matches | Fold change (IBS512 vs. UA159) | P (t test) (IBS512 vs. UA159) | Alteration in IBS512 vs. UA159a |
|---|---|---|---|---|---|
| SMU.548 | UDP-N-acetylmuramoyl-l-alanyl-d-glutamate synthetase | 4/2 | −2.0/−1.7 | 0.043/0.032 | Downregulated |
| SMU.644 | Putative competence protein CoiA | 3 | −6.4 | 0.001 | Downregulated |
| SMU.650 | Alanyl-tRNA synthetase | 10 | −11.9 | 0.008 | Downregulated |
| SMU.858 | Aspartate carbamoyltransferase subunit | 5 | −11.9 | 0.008 | Downregulated |
| SMU.878 | Multiple sugar binding ABC transporter | 7 | −2.3 | 0.045 | Downregulated |
| SMU.910 | Glucosyl-S-transferase | 8/11/15 | −1.8/−2.1/−4.3 | 0.031/0.042 | Downregulated |
| SMU.962 | Putative dehydrogenase | 2 | −2.1 | 0.042 | Downregulated |
| SMU.1032 | Putative transposon integrase | 9 | −2.4 | 0.021 | Downregulated |
| SMU.1293 | Hypothetical protein | 3 | −1.9 | 0.017 | Downregulated |
| SMU.1334 | Putative phosphopantetheinyl transferase | 7 | −6.9 | 0.019 | Downregulated |
| SMU.1342 | Putative bacitracin synthetase 1; BacA | 10 | −4.6 | 0.031 | Downregulated |
| SMU.1462 | Putative oxidoreductase | 3 | −1.9 | 0.045 | Downregulated |
| SMU.1500 | Putative exonuclease RexB | 4 | −4.6 | 0.031 | Downregulated |
| SMU.1509 | Putative transcriptional regulator | 3 | −1.8 | 0.030 | Downregulated |
| SMU.1657 | Putative nitrogen regulatory protein PII | 2 | −14.8 | 0.001 | Downregulated |
| SMU.1949 | Putative membrane carboxypeptidase, penicillin-binding protein 2A | 6 | −1.9 | 0.017 | Downregulated |
| SMU.1997 | Putative ComX1; transcriptional regulator | 3 | −7.7 | 0.002 | Downregulated |
| SMU.2067 | Putative stress response protein; possible cell wall biogenesis protein | 2/4 | −2.5/−6.4 | 0.034/0.001 | Downregulated |
| SMU.38 | Putative transcriptional regulator | 1 | 6.0 | 0.179 | Upregulated |
| SMU.82 | Molecular chaperone DnaK | 15 | 2.5 | 0.011 | Upregulated |
| SMU.374 | Putative oxidoreductase | 3 | 2.2 | 0.007 | Upregulated |
| SMU.475 | Hypothetical protein | 3 | 3.8 | 0.029 | Upregulated |
| SMU.491 | Putative DeoR-type transcriptional regulator | 1 | 3.4 | 0.062 | Upregulated |
| SMU.552 | Cell division protein FtsZ | 3 | 16.5 | 0.001 | Upregulated |
| SMU.605 | Hypothetical protein | 3 | 6.0 | 0.179 | Upregulated |
| SMU.640 | Putative transcriptional regulator (GntR family) | 3/2/3 | 1.7/9.5/16.5 | 0.029 | Upregulated |
| SMU.923 | Putative ABC transporter; ATP-binding protein | 3 | 2.0 | 0.031 | Upregulated |
| SMU.1241 | Exonuclease ABC subunit C (UvrC; nucleotide excision repair) | 6 | 3.1 | 0.058 | Upregulated |
| SMU.1810 | Putative bacteriocin operon component | 1 | 1.7 | 0.004 | Upregulated |
| SMU.2075 | Hypothetical protein | 5 | 3.1 | 0.034 | Upregulated |
a
The up- and downregulated proteins were identified in S. mutans IBS512 and UA159, respectively.