TABLE 2.
Detailed characterization of the putative transformants and other isolates from the same PFGE cluster
| Set | PFGE profile (cluster) | Isolate | Serotype | MLST (ST) | Surface protein gene profile | Sequencinga |
RFLP (cpsG-neuA) | Hybridization-positive fragment size (kb)b | Hybridization probeb | Pilus island gene profile | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| cpsD-cpsG | neuB-neuA | ||||||||||
| S1 | Identical (G) | SH366 | II | 24 | bca | II | II | II | 450 | II | PI-2a |
| 707618 | Ia | 24 | bca | Ia | Ia | Ia | 450 | Ia | PI-2a | ||
| 12631 | Ia | 24 | bca | Ia | Ia | Ia | 450 | Ia | PI-2a | ||
| S2 | Identical (I) | CN4609 | Ia | 10 | bca + bac | Ia | Ia | Ia | 582 | Ia | PI-1 + PI-2a |
| 275586 | Ib | 10 | bca + bac | Ib | Ib | Ib | 582 | Ib | PI-1 + PI-2a | ||
| 321927 | Ib | 10 | bca + bac | Ib | Ib | Ib | 582 | Ib | PI-1 + PI-2a | ||
| S3 | Identical (G) | 312754 | Ib | 24 | bca | Ia | Ia | Iac | 436.5 | Ia | PI-2a |
| 333425 | Ia | 24 | bca | Ia | Ia | Ia | 436.5 | Ia | PI-2a | ||
| 401936 | Ia | 24 | bca | Ia | Ia | Ia | 436.5 | Ia | PI-2a | ||
| S4 | Identical (M1) | 316560 | III | 295 | rib + bac | III | III | III | 500 | III | PI-1 + PI-2a |
| 768348 | II | 28 | rib + bac | II | II | II | 500 | II | PI-1 + PI-2a | ||
| 850409 | II | 28 | rib + bac | II | II | II | 500 | II | PI-1 + PI-2a | ||
| S5 | One-band difference (B) | 406301 | IV | 2 | eps | IV | IV | IV | 300 | IV | PI-1 + PI-2a |
| 424176 | V | 1 | alp3 | V | V | V | 300 | V | PI-1 + PI-2a | ||
| 345352 | V | 1 | alp3 | V | V | V | 300 | V | PI-1 + PI-2a | ||
| S6 | Three-band difference (C) | 351726 | IV | 2 | eps | IV | IV | IV | 300 | IV | PI-1 + PI-2a |
| 314460 | V | 2 | eps | V | V | V | 339.5 | V | PI-1 + PI-2a | ||
| 371990 | V | 2 | eps | V | V | V | 339.5 | V | PI-1 + PI-2a | ||
| S7 | Three-band difference (G) | 927045 | V | 23 | eps | V | V | V | 582 | V | PI-2a |
| 401207 | Ia | 23 | eps | Ia | Ia | Ia | 582 | Ia | PI-2a | ||
| 314927 | Ia | 23 | eps | Ia | Ia | Ia | 582 | Ia | PI-2a | ||
| S8 | Three-band difference (P) | 931422 | V | 19 | eps + bac | V | V | V | 582 | V | PI-1 + PI-2a |
| 950541 | III | 19 | rib | III | III | III | 582 | III | PI-1 + PI-2a | ||
| 880372 | III | 19 | rib | III | III | III | 582 | III | PI-1 + PI-2a | ||
| S9 | Three-band difference (G) | 2227 | III | 396 | rib | III | III | III | 535.5 | III | PI-1 + PI-2a |
| SH436 | Ia | 24 | bca | Ia | Ia | Ia | 500 | Ia | PI-2a | ||
| 342399 | Ia | 24 | bca | Ia | Ia | Ia | 500 | Ia | PI-2a | ||
a
The capsular type compatible with the single nucleotide polymorphisms and insertions found using the methodology described in the text is indicated.
b
That is, the size of the fragment in the PFGE profile that presented a positive hybridization signal and the corresponding serotype identified by the probe (see the text).
c
One fragment different from the characteristic serotype Ia RFLP profile.