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Glucose depletion (A) and acetate accumulation and depletion (B) in the culture supernatants of wild-type strains SA564 and UAMS-1 and aconitase SA564 acnA::tetM and UAMS-1 acnA::tetM mutant strains, plotted as a function of time. Bacterial cultures were grown in tryptic soy broth (Difco) at 37°C, with a flask-to-medium ratio of 10:1, and aerated at 225 rpm. The results are representative of three independent experiments.
