TABLE 1.
List of strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| H37Rv | Laboratory strain (ATCC 27294) of M. tuberculosis H37Rv | |
| ΔrelE1 strain | relE1 mutant strain of M. tuberculosis H37Rv | This work |
| ΔrelE2 strain | relE2 mutant strain of M. tuberculosis H37Rv | This work |
| ΔrelE2 attB::relE2 strain | relE2::hyg complemented with relE2 at the attB site | This work |
| ΔrelE3 strain | relE3 mutant strain of M. tuberculosis H37Rv | This work |
| ΔmazF1 strain | mazF1 mutant strain of M. tuberculosis H37Rv | This work |
| ΔmazF5 strain | mazF5 mutant strain of M. tuberculosis H37Rv | This work |
| ΔmazF6 strain | mazF6 mutant strain of M. tuberculosis H37Rv | This work |
| Plasmids | ||
| p2NIL | Cloning vector, Kmr | 45 |
| pGOAL17 | Plasmid carrying sacB and lacZ genes as a PacI fragment, Apr | |
| p2NrelE1KO | p2NIL vector carrying upstream and downstream regions of relE1, the hyg cassette, and the PacI cassette from pGOAL17 | This work |
| p2NrelE2KO | p2NIL vector carrying upstream and downstream regions of relE2, the hyg cassette, and the PacI cassette from pGOAL17 | This work |
| p2NrelE3KO | p2NIL vector carrying upstream and downstream regions of relE3, the hyg cassette, and the PacI cassette from pGOAL17 | This work |
| pCR2.1 | Cloning vector, Kmr Apr | Invitrogen |
| pExp5-NT | T7-based expression vector | Invitrogen |
| pNT-RelE1 | pExp5-NT carrying relE1 | This work |
| pNT-RelBE1 | pExp5-NT carrying relB1 and relE1 | This work |
| pNT-RelE2 | pExp5-NT carrying relE2 | This work |
| pNT-RelBE2 | pExp5-NT carrying relB2 and relE2 | This work |
| pNT-RelE3 | pExp5-NT carrying relE3 | This work |
| pNT-RelBE3 | pExp5-NT carrying relB3 and relE3 | This work |
| pTE-mcs-1 | E. coli-Mycobacterium shuttle vector, Hygr, Atc inducible in M. tuberculosis | Kind gift from Sabine Ehrt; 11 |
| pTE-relE1 | pTE-mcs-1 carrying relE1 | This work |
| pTE-relE2 | pTE-mcs-1 carrying relE2 | This work |
| pTE-relE3 | pTE-mcs-1 carrying relE3 | This work |
| pTE-mazF1 | pTE-mcs-1 carrying mazF1 | This work |
| pTE-mazF2 | pTE-mcs-1 carrying mazF2 | This work |
| pTE-mazF3 | pTE-mcs-1 carrying mazF3 | This work |
| pTE-mazF4 | pTE-mcs-1 carrying mazF4 | This work |
| pTE-mazF5 | pTE-mcs-1 carrying mazF5 | This work |
| pTE-mazF6 | pTE-mcs-1 carrying mazF6 | This work |
| pTE-mazF7 | pTE-mcs-1 carrying mazF7 | This work |