Skip to main content
. 2009 Dec 28;192(5):1377–1386. doi: 10.1128/JB.01448-09

FIG. 5.

FIG. 5.

Proton translocation mutants in a linked Mdt complex. (A) Introduction of a proton translocation mutation into an artificial gene for a linked MdtB-C-BHis complex harbored in pHMA-BCB10. To express a linked Mdt complex having only one mutant MdtB subunit, D410A mutation was introduced in either one of the two mdtB subunit sequences of the artificial gene. Similarly, D401A mutation was introduced into the mdtC sequence. X represents the location of these mutations in the proton translocation pathway. (B) Activities of proton translocation mutants of a linked Mdt B-C-BHis trimer in the BL21KAMR host. Efflux activities were estimated from the levels of resistance to cloxacillin by using the gradient plate method. Error bars show standard deviations. (C) Expression level of proton translocation mutants of a linked Mdt B-C-BHis trimer. The linked Mdt trimers were analyzed by Western blotting using an anti-His-tag antibody.