FIG. 7.

Comparative rates of Axe2 degradation in SH1000 wild-type and clp mutant strains with an alternate HA epitope tag. Wild-type (A), ΔclpB (B), ΔclpC (C), and ΔclpP (D) strains were transformed with pEPSA5::axe2_C-HA (pALC6682). At the top of each panel is a Western blot from the respective time points using a primary α-HA antibody. The graphs depict the percentage of signal at each time point compared to t = 0. The cells were analyzed like those in Fig. 4. The experiments were repeated twice, and representative blots are shown.