TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Characteristic(s) | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | Common cloning strain | 9 |
| E. coli SM-10 | Donor strain in conjugation experiments | 22 |
| E. coli BL21(DE3) | High-level expression by IPTG induction of T7 RNA polymerase from lacUV5 promoter | 27 |
| R. rubrum Wt (Str-2) | Wild-type strain, streptomycin resistant | 10 |
| R. rubrum cbbM (I19A) mutant | RubisCO disruption strain | 10 |
| R. rubrum rlpA mutant | RLP disruption strain | This study |
| R. rubrum cbbM rlpA mutant | RubisCO/RLP double disruption strain | This study |
| Plasmids | ||
| pTBRrRLP | R. rubrum rlpA gene cloned into pCRTOPOBLUNT | This study |
| pTBRLPGm | Gentamicin gene inserted into StuI site of pTBRrRLP | This study |
| pTB-MPI | R. rubrum mtrI gene cloned into pCRTOPOBLUNT | This study |
| pTB-MPIGm | Gentamicin gene inserted into mtrI gene in pTB-MPI | This study |
| pUC1318Gm | Source of Gm cartridge | 1 |
| pSUP202 | Suicide vector | 22 |
| pSUP-RLPGm | Disrupted rlpA gene from pTBRLPGm cloned into PstI site of pSUP202 | This study |
| pSUP-MPIGm | Disrupted mtrI gene from pTB-MPIGm cloned into EcoRI site of pSUP202 | This study |
| pRK415 | Broad-host-range vector | 16 |
| pRPR | pRK415 vector containing R. rubrum RLP promoter region | This study |
| pRP-RRLP | R. rubrum rlpA gene cloned into pRPR | This study |
| pRP-CRLP | C. tepidum RLP gene cloned into pRPR | This study |
| pRP-BRLP | B. subtilis RLP gene cloned into pRPR | This study |
| pRP-PRLP1 | R. palustris RLP1 gene cloned into pRPR | This study |
| pRP-PRLP2 | R. palustris RLP2 gene cloned into pRPR | This study |
| pRPS-MCS3 | Broad-host-range plasmid containing R. rubrum cbbM promoter and cbbR gene | 24 |
| pRPS-RrcbbM | R. rubrum cbbM gene cloned into pRPS-MCS3 | This study |
| pRPS-RpcbbM | R. palustris cbbM gene cloned into pRPS-MCS3 | Satagopan and Tabita, unpublished |