FIG. 5.

RAB3D and RAB26 expression are zymogenic cell specific and MIST1 dependent in vivo. Images of in situ hybridization of mouse fundic gastric gland sections showing antisense RAB3D (A) and RAB26 (B) signals confined to the ZCs at the base of the glands are shown. Note the absence of signal in parietal cells (arrowheads in the higher-magnification view below each main panel). (C) Results of quantitative RT-PCR of ZCs dissected by LCM from Mist1^−/− mice. RAB3D and RAB26 expression levels are significantly decreased in these mice. Note that the y axis values are expressed as log₂ values relative to levels in wells containing no cDNA following 18S rRNA normalization; hence, zero indicates essentially no detectable levels of given amplicon. RAB26 expression in the Mist1^−/− mice is nearly completely abrogated. Primers for another potential MIST1 target, CCPG1, indicate no significant change in expression (n.s.), indicating that the loss of MIST1 specifically affects levels of the two RABs. WT, wild type.