Skip to main content
. 2009 Dec 22;30(5):1171–1181. doi: 10.1128/MCB.01500-09

FIG. 2.

FIG. 2.

Loss of HDAC1 in a p21-deficient background rescues impaired proliferation of HDAC1-null ES cells. (A) Phenotype of HDAC1+/+ and HDAC1−/− embryos in a p21 null background. Embryos were obtained from HDAC1+/− p21−/− crossbreeding at E8.5 and E9.5. The genotype of the embryos was visualized by in situ hybridization with an HDAC1 riboprobe. (B) Immunoblot analysis of HDAC1 and p21 protein expression. Expression of HDAC1 and p21 was examined in protein extracts prepared from HDAC1+/+ p21+/+, HDAC1−/− p21+/+, HDAC1+/+ p21−/−, and HDAC1−/−p21−/− ES cell lines. (C) Growth curves of HDAC1+/+ p21+/+, HDAC1−/− p21+/+, HDAC1+/+ p21−/−, and HDAC1−/−p21−/− ES cell lines. For each cell line, 105 cells were seeded in triplicates, and aliquots were counted daily during a time period of 4 days and shown as mean values ± the SD.