FIG. 2.

Virus replication is reduced in ICP27 phosphorylation site mutant infection. (A) Vero cells were infected at an MOI of 1 with wild-type KOS, the null mutant 27-GFP, and the indicated alanine substitution mutants. Samples were harvested at 0, 4, 8, 16, and 24 h after infection. Virus titers were determined by plaque assays. (B) Vero cells were infected as described for panel A with wild-type KOS, 27-GFP, and the glutamic acid substitution mutants as indicated. Samples were harvested and assayed at 0, 4, 8, 16, and 24 h after infection as described for panel A. The experiments were performed three times, and error bars showing the standard deviations are shown.