FIG. 3.

Rescued phosphorylation site mutant viruses grow as well as wild-type KOS. (A) Vero cells were infected with KOS, N-YFP-ICP27, and the rescued viruses S16AR, S114AR, and S16,18,114ER at an MOI of 1. Samples were harvested at 0, 4, 8, 16, and 24 h after infection. Virus titers were determined by plaque assays on Vero cells. The experiment was repeated twice, and averages are shown. (B) Vero cells were infected with KOS, N-YFP-ICP27, and mutants S16A, S114A, S16,18,114A, and S16,18,114E at an MOI of 1, and samples were harvested at 4, 8, 16, and 24 h after infection. Virus titers were determined by assay on Vero cells. The experiments were performed twice, and the averages are shown. (C) Vero 2-2 cells were infected with KOS, N-YFP-ICP27, S16A, S114A, S16,18,114A, and S16,18,114E at an MOI of 1, and samples were harvested at 4, 8, 16, and 24 h after infection. Virus yields were determined by plaque assays on 2-2 cells. The experiments were performed twice, and the averages are shown.