FIG. 8.

ICP27 does not colocalize with RNAP II in cells infected with phosphorylation site mutants. HeLa cells were infected with wild-type KOS, S16E, and S16,18,114E at an MOI of 5 for 4 and 8 h as indicated. Cells were fixed, and immunofluorescent staining was performed with anti-RNAP II antibody ARNA3 and anti-ICP27 antibody. Images were visualized with a Zeiss Axiovert S100 microscope at a magnification of ×100. Arrows point to the areas of colocalization in wild-type KOS at 4 h after infection and to areas where colocalization was not seen in the zoomed images for S16E at 4 h and S16,18,114E at 8 h.