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. 2009 Dec 16;84(5):2223–2235. doi: 10.1128/JVI.02090-09

FIG. 6.

FIG. 6.

The cysteine residues in Sf-Stk and gp55 are required for Sf-Stk activation and mitogen-activated protein (MAP) kinase signaling. (A) 293 cells were transfected with myc-tagged wild-type or mutant forms of Sf-Stk with wild-type or mutant gp55. Cell lysates were immunoprecipitated with antiphosphotyrosine antibody and blotted with anti-myc antibody. (B) 293 cells were transfected with myc-tagged wild-type Sf-Stk or Sf-StkC4A with wild-type gp55 or gp55C4A. Cell lysates were blotted with anti-phosho-Erk1/2 antibody, stripped, and reprobed with anti-Erk1/2 antibody. (C) 293 cells were transfected with myc-tagged wild-type Sf-Stk or Sf-StkC4A and wild-type gp55 or gp55C4A in the presence of Ap-1 luciferase and Renilla reporter plasmids. At 48 h after transfection, luciferase assays were performed according to the manufacturer's instructions. *, P < 0.01; **, P < 0.01. Error bars indicate standard deviations.