FIG. 8.

gp55 and cysteines regulate the cell surface localization of Sf-Stk (A) CHO cells were transfected with myc-Sf-Stk and gp55 or empty vector. Forty-eight hours later, cell surface proteins were biotinylated and isolated. The cell surface proteins were separated by SDS-PAGE. Membranes were blotted with anti-myc antibody. (B) 293 cells were transfected with EGFP, myc-Sf-Stk, and gp55, gp55C4A, or empty vector. Forty-eight hours later, cells were resuspended and stained with Alexa Fluor 647-conjugated myc tag antibody. Cells were washed and analyzed by flow cytometry. EGFP expression populations were gated as transfected cells.