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. 2009 Dec 23;84(5):2533–2546. doi: 10.1128/JVI.01909-09

FIG. 1.

FIG. 1.

EBV episomes are localized in perichromatic regions of the nucleus. Immuno-FISH image of HEK293-EBV+ (A) and Raji (C) cells displaying a DAPI DNA counterstain (blue; channel 2) and a fluorescence in situ hybridization of EBV genomic DNA (red). 3D reconstruction of HEK293-EBV+ (B) and Raji (D) treated with hypertonic buffer with an enlarged section. EBV DNA is detected near the condensed chromatin, which defines the perichromatic localization. EBV DNA neither colocalizes with the chromatic domain nor exists unassociated with the chromatin. Enlargements are indicated by white-lined squares. 3D reconstructions were used to quantify the perichromatic localization of EBV genomes. Scale bar = 2 μm. Signal intensity scans for EBV (red; channel 0) and DNA counterstain (blue; channel 1) along the indicated line and direction in HEK293-EBV+ (E) and Raji (F) confirm the perichromatic localization. Localization of EBV is not observed in the peaks of the DNA counterstain but is observed in the shoulders of DAPI-stained regions. Line scans represent one selected confocal plane, which might result in unambiguous results. The asterisk indicates a signal that seems unassociated in this particular confocal plane but is clearly associated in an adjacent plane. See also Fig. S2 in the supplemental material for details. Scale bar = 2 μm.