TABLE 1.
Localization analysis of the different cell linesa
| Type of localization | % with result for cell line |
|||||
|---|---|---|---|---|---|---|
| Raji (n = 23) | HEK293 EBV+ (n = 42) | 2908 wt-oriP (n = 26) | 2910ΔDS (n = 28) | 2912eFR (n = 29) | 2913eDS (n = 30) | |
| Perichromatic localization | 95.7 | 85 | 84.6 | 96.4 | 69.6 | 93.3 |
| Chromatic localization | 0 | 0 | 0 | 0 | 0 | 0 |
| Interchromatic localization | 4.3 | 0 | 0 | 0 | 0 | 0 |
| Cytosolic localization | 0 | 0 | 0 | 0 | 15.2 | 0 |
| No signal | 0 | 15.0 | 15.4 | 3.6 | 15.2 | 6.7 |
a
EBV genomes localize predominantly in the perichromatin. Cells showing no signal for EBV-DNA were included in the statistics to take the number of cells into account that have lost the EBV genome. Signals qualify for chromatic localization if signals for EBV-FISH and DAPI signals completely overlap, which was not observed (see also Fig. S2 in the supplemental material). EBV signals score for perichromatin if the FISH signal localizes at the border of the chromatin-dense region and a partial overlap of both signals is observed. There was no association of both signals in the interchromatin.