FIG. 5.

ICP27 and TAP/NXF1 do not colocalize in infections with phosphorylation site mutants. HeLa cells were transfected with pEGFP-TAP and were subsequently infected with HSV-1 KOS, S16E, or S16,18,114E. Cells were fixed at 4 and 8 h after infection and immunostained with anti-ICP27 antibody. GFP fluorescence was visualized directly. (A) Images were captured with a Zeiss Axiovert S100 microscope. Magnification, ×100. (B) Images were viewed with an LSM 510 confocal microscope. Magnification, ×63. White arrows point to GFP-TAP sites, and yellow arrows mark ICP27 sites in the merged images.