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Colocalization with MeCP2 requires multiple regions of LANA. (A) NIH 3T3 cells were transfected with plasmids encoding DsRed-MeCP2FL and the following GFP-LANA derivatives: GFP-LANAFL (a and b), GFP-LANAFLΔ22 (c and d), GFP-LANAN (e and f), GFP-LANANΔ22 (g and h), and GFP-LANAC (i and j). (B) Fusion of the CBM to LANAC is not sufficient for colocalization with MeCP2. Cells were transfected to express GFP-LANACBM+C on its own (a and b) or with DsRed-MeCP2FL (c and d).
