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LANA binds to the multiple domains within MeCP2. In vitro binding analysis using recombinant GST-LANAC purified from bacteria and 35S-labeled full-length or truncated MeCP2 (shown schematically below) synthesized by in vitro translation. Binding reactions were performed with 100 mM NaCl, and mixtures were washed in 250 mM NaCl. 35S-labeled LANAC (lanes 6, 12, and 18) served as a positive control for binding.
