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. 2009 Dec 23;84(5):2597–2609. doi: 10.1128/JVI.02256-09

FIG. 1.

FIG. 1.

Immunoblot analysis of viral glycoproteins in HCMV TR- and AD169-infected cells and extracellular virus particles. Human fibroblasts (NHDF) were infected with 2 IU per cell of TR or AD169 for 7 days. Extracellular viral particles were purified from clarified culture supernatants by centrifugation though 20% sorbitol cushions (V). Virus particles and infected cells (C) were extracted with 1% Triton X-100 and analyzed by Western blotting using mouse MAb specific for gB (27-156) or calnexin or rabbit polyclonal antibodies specific for gH, gL, TR gO (TR gO254 produced against gO residues 254 to 271), AD169 gO, or UL130. Note that the TR gO254 antibodies do not recognize AD169 gO, and AD169 gO antibodies do not recognize TR gO; thus, separate blots are shown. A longer exposure of the TR gO blot was provided to show the very small quantity of TR gO in virus particles. Numbers below each lane indicate the relative abundance of each protein. Shown are representative results from three independent experiments. Molecular weight markers are shown along the left side of each panel.