FIG. 3.

Pulse-chase analysis of the maturation of N-linked oligosaccharides associated with gO and gH in TR-infected cells versus AD169-infected cells. Human fibroblasts (NHDF) were infected for 4 days with 3 IU/cell of TR (A and C) or AD169 (B and D). Cells were labeled for 15 min with [³⁵S]methionine/cysteine and then label chased for 45, 120, or 240 min. Cell extracts were analyzed by immunoprecipitation (IP) with anti-TR gO254 antibodies (A), anti-AD169 gO antibodies (B), or anti-gH MAb 14-4b (C and D). Immunoprecipitated proteins were either left untreated or treated with endo H or PNGase F. Arrows indicate bands corresponding to gO, gH, and gL, and faster-migrating forms of these proteins after oligosaccharides were removed by endo H or PNGase F (H, F). Molecular weight markers are shown along the left side of each panel.