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. 2009 Dec 23;84(5):2597–2609. doi: 10.1128/JVI.02256-09

FIG. 5.

FIG. 5.

Expression of TR gO by nonreplicating Ad vectors. U373 cells were infected with various Ad vectors for 16 to 24 h and labeled for 2 h with [35S]methionine/cysteine, and then expressed HCMV proteins were immunoprecipitated and analyzed by gel electrophoresis. (A) Cells were infected with 50 PFU/cell of an Ad vector expressing TR gO or with Ad vectors expressing gH, gL, and gO (50 PFU/cell each). gO was immunoprecipitated using rabbit TR gO254 antibodies in the presence or absence of gO 254-271 peptide as a competitive inhibitor. (B) Cells were infected with 50 PFU/cell of either Ad TR gO or an Ad vector containing a codon-optimized TR gO gene, AdTRgO(co). gO was immunoprecipitated using anti-TR gO254 antibodies with or without peptide. The two panels represent samples analyzed on the same gel. (C) Cells were infected with Ad vectors expressing gH and gL or gH, gL, and AdTRgO(co). gH was precipitated using anti-gH MAb 14-4b, gL with rabbit anti-gL antibodies, or gO precipitated with rabbit anti-TR gO254 antibodies. Molecular weight markers are shown along the left side of each panel.