FIG. 6.

Maturation of N-linked oligosaccharides associated with gH and gO expressed by Ad vectors. U373 cells were infected with the indicated combinations of Ad vectors expressing gH, gL, TR gO, UL128, UL130, and UL131. Cells were labeled for 5 min with [³⁵S]methionine/cysteine, and then the label was chased for 0, 60, or 180 min. (A) gH was immunoprecipitated with anti-gH MAb 14-4b; (B) gO was immunoprecipitated using TR gO254 antibodies. Immunoprecipitated proteins were either left untreated or treated with endo H. Arrows indicate proteins corresponding to gH, gO, or endo H-sensitive forms of these proteins (H). Molecular weight markers are shown along the left side of each panel.