TABLE 2.
Activity of GRFT on the cytopathic effects of selected coronavirusesa
| Virus, host, and treatment compound | Cytopathic effect assay |
Neutral red uptake assay |
||||
|---|---|---|---|---|---|---|
| EC50 | IC50 | SI | EC50 | IC50 | SI | |
| BCoV in human ileocecal colorectal human adenocarcinoma cells (HRT-18G) | ||||||
| GRFT | 0.057 | 32 | 560 | <0.032 | >100 | >3,100 |
| Ribavirin | 0.32 | 50 | 313 | <0.32 | 30 | 94 |
| IBV (Connecticut A5968) in chicken embryonic fibroblast cells (UMNSAH/DF-1) | ||||||
| GRFT | <0.032 | 68 | >2,100 | <0.032 | >100 | >3,100 |
| Multiferon | 0.0,012 | >0.03 | >25 | 0.0,029 | >0.03 | >10 |
| MHV (JHM) in mouse embryonic liver cells (BNL) | ||||||
| GRFT | 0.23 | 0.44 | 1.9 | 0.13 | 2.5 | 19 |
| Multiferon | 0.001 | >0.3 | >290 | 0.0,012 | >0.3 | >260 |
| PCoV (Miller) in pig testis fibroblast cells (ST) | ||||||
| GRFT | 0.57 | 57 | 100 | 0.77 | 46 | 59 |
| Multiferon | 0.000,095 | >0.03 | >320 | 0.00,017 | >0.03 | >180 |
| HCoV (OC43) in human ileocecal colorectal human adenocarcinoma cells (HCT-8) | ||||||
| GRFT | 0.16 | 52 | 320 | 0.048 | >100 | >2,100 |
| Multiferon | 0.0,006 | >0.03 | >50 | 0.0,008 | >0.03 | >37 |
| HCoV (229E) in human diploid fibroblast cells (MRC-5) | ||||||
| GRFT | 0.18 | >10 | >56 | 0.33 | >10 | >30 |
| Ribavirin | 18 | >1,000 | >56 | 26 | >1,000 | >38 |
| HCoV (NL63) in rhesus monkey kidney cells (LLC-MK2) | ||||||
| GRFT | <0.0,032 | 10 | >3,100 | <0.0,032 | >10 | >3,100 |
| Ribavirin | 0.63 | 320 | 500 | 1.6 | 120 | 77 |
a
EC50s and IC50s are shown in micrograms per milliliter for GRFT and ribavirin and in international units per milliliter for multiferon.