FIG. 3.

Caspase-cleaved MAVS loses the ability to activate the IFN promoter. N18 cells were cotransfected with p125-Luc (0.3 μg), IRF-3/pCR3.1 (0.3 μg), pRL-TK (0.1 μg), and the indicated MAVS constructs (0.6 μg) for 24 h, and the cell lysates were harvested and analyzed by dual-luciferase assay and by Western blotting, using antibodies against Flag, Ser396-phosphorylated IRF-3, IRF-3, and actin, as indicated at the left. Firefly luciferase activity was normalized to that of Renilla luciferase, and the change in induction relative to that of the green fluorescent protein (GFP) control was determined. The results are expressed as averages and standard deviations (n = 3 per group).