FIG. 7.

MAVS- and virus-induced IFN signaling is attenuated in MAVS knockdown cells. (A) Lentivirus-transduced A549 cells with (+) or without (−) MAVS-targeting shRNA were transfected with p125-Luc, IRF-3/pCR3.1, pRL-TK plus GFP, or MAVS as described in the legend to Fig. 3. For DEN-2-induced IFN-β promoter analysis, the cells were adsorbed with DEN-2 (MOI = 5) for 2 h and then transfected with p125-Luc (0.6 μg) and pRL-TK (0.15 μg) for 24 h. The change in IFN-β promoter activation was determined as described in the legend to Fig. 3. The IFN-β induction of the indicated groups was compared by two-tailed Student's t test. (B and C) Cells were prepared as described for panel A, and the cell lysates were analyzed by Western blotting with the antibodies indicated at the left.