FIG. 9.

Knockdown of MAVS attenuates DEN-2-triggered MMP disruption. (A) Mock- or DEN-2-infected (MOI of 5) cells were cultured in the presence or absence of z-VAD-fmk (100 μM) for 48 h and then analyzed by flow cytometry for JC-1 and annexin V double staining, as described in the legend to Fig. 5B. (B) The cell lysates described for Fig. 1G were analyzed with the antibodies indicated at the left. (C) Control (shLacZ) and MAVS knockdown (shMAVS) cells were mock infected or infected with DEN-2 (MOI of 5) and transfected with dnIRF-3-GFP or control GFP vector. Cells were harvested at 48 h p.i. and stained with Cy5-conjugated annexin V. GFP-positive cells were gated and analyzed for annexin V positivity by flow cytometry.