FIG. 1.
Reactivities of antibodies to different coated glucans. Reactivity is expressed as 1,000 divided by the coating concentration (μg/ml) that is needed to achieve an OD of 1. Starting reactions not yet reaching an OD of <1 at 16 μg/ml were assigned a cOD = 1 of 32 μg/ml, while glucans not showing any reactivity at 16 μg/ml were assigned a cOD = 1 of 1,000. BAK, baker's yeast glucan; BAR, barley glucan; CUR, curdlan; LAM, laminarin; LIC, lichenan; OAT, oat glucan; PAC, pachyman; PAR, paramylon; PUL, pullulan; PUS, pustulan; SCH, schizophyllan; SCL, scleroglucan; XYL, xyloglucan. (A) Polyclonal rabbit anti-laminarin IgG (U1); (B) biotinylated monoclonal mouse antilaminarin IgG (B2); (C) anti-β-(1,3)-(1,6)-glucan mouse monoclonal IgM (P1); (D) anti-scleroglucan rabbit polyclonal Ig (P2).