TABLE 2.
Frequencies of maintenance of phage P1kc and T4GT7 genes in Enterobacteriaceae strains
| Phage | Recipient | Result for infection range indicator: |
Increase in total no. of cellsc | Increase in phage gene copy no. (SD)d | Maintenance frequency (SD) as determined bye: |
||
|---|---|---|---|---|---|---|---|
| Plaque formationa | Production of progenyb | Real-time PCR | CPRINS-FISH | ||||
| P1kc | C. freundii | − | − | 7.0 × 103 | None | <1.5 × 10−3 | ND |
| E. aerogenes | + | + | 1.7 × 103 | 7.7 × 103 (6.5 × 103) | 5.0 × 100 (4.2 × 100) | ND | |
| E. coli | + | + | 7.2 × 103 | 5.5 × 103 (2.7 × 103) | 9.1 × 10−1 (0.5 × 10−1) | ND | |
| P. mirabilis | − | − | 7.4 × 103 | None | <1.5 × 10−3 | ND | |
| S. Enteritidis | − | − | 8.4 × 103 | None | <1.7 × 10−4 | ND | |
| Y. enterocolitica | − | − | 4.6 × 103 | None | <1.8 × 10−4 | ND | |
| T4GT7 | C. freundii | − | − | 1.5 × 103 | 7.5 × 103 (4.0 × 103) | 8.3 × 10−1 (4.4 × 10−1) | 8.6 × 10−2 (3.4 × 10−2) |
| E. aerogenes | + | + | 8.7 × 102 | 1.2 × 103 (0.8 × 103) | 8.0 × 10−1 (5.0 × 10−1) | 4.0 × 10−1 (0.7 × 10−1) | |
| E. coli | + | + | 1.1 × 104 | 7.0 × 104 (2.7 × 104) | 8.0 × 101 (3.0 × 10) | 2.1 × 10−1 (0.4 × 10−1) | |
| P. mirabilis | − | − | 4.0 × 103 | 5.8 × 103 (4.2 × 103) | 3.3 × 10−1 (2.4 × 10−1) | 3.4 × 10−2 (2.2 × 10−2) | |
| S. Enteritidis | − | − | 1.0 × 104 | 7.6 × 101 (5.0 × 101) | 1.0 × 10−2 (0.7 × 10−2) | 8.8 × 10−2 (2.0 × 10−2) | |
| Y. enterocolitica | − | − | 3.6 × 103 | 1.6 × 104 (0.4 × 104) | 6.1 × 10−1 (1.6 × 10−1) | 2.2 × 10−2 (2.9 × 10−2) | |
Plaque formation on soft agar was tested.
The production of progeny phage particles was observed via epifluorescence microscopy.
The increase (n-fold) in the total number of cells during bacterial growth for 24 h was determined via epifluorescence microscopy.
The increase (n-fold) in the copy number of the phage tail fiber gene during bacterial growth for 24 h was determined by real-time PCR. Values in parentheses indicate standard deviations of results for triplicate samples.
Maintenance frequencies were determined by real-time PCR and CPRINS-FISH analyses targeting the phage tail fiber gene and are shown as the phage tail fiber gene copy numbers per bacterial genome and the numbers of gene-positive cells divided by the total numbers of cells, respectively. Values in parentheses indicate standard deviations of results for triplicate samples. ND, not determined.