TABLE 2.

Average SNR of reference probe and target^a

Reference probe amt (%)	Reference target concn (fmol/μl)
	12.5	1.25	0.625	0.25	0.125	0.0625	0.025	0.0125
10.00	115.5 (80.1)	67.2 (99.1)	52.3 (98.9)	19.5 (100)	12.2 (100)	4.67 (96.4)	2.16 (39.1)	0.9 (6.6)
5.00	97.0 (91.4)	52.3 (100)	36.5 (100)	15.5 (100)	5.9 (97.8)	4.7 (91.8)	1.1 (9.5)	0.8 (7.3)
2.50	64.8 (97.8)	36.1 (100)	28.8 (100)	12.1 (100)	6.9 (98.1)	3.1 (71.0)	1.5 (21.5)	0.7 (18.9)
1.00	35.6 (100)	18.1 (100)	15.4 (100)	6.8 (94.4)	2.6 (39.0)	2.0 (35.8)	0.7 (4.7)	0.5 (2.0)
0.50	23.9 (100)	13.8 (100)	8.7 (99.4)	4.9 (83.0)	2.1 (6.2)	1.3 (14.5)	0.6 (3.1)	0.5 (0.4)
0.25	13.8 (100)	7.6 (95.0)	4.9 (89.7)	2.7 (44.0)	1.1 (9.7)	0.9 (6.8)	0.4 (0)	0.4 (0.2)
0.10	6.2 (91.2)	3.4 (54.7)	2.3 (40.3)	1.3 (15.4)	0.7 (3.8)	0.6 (3.1)	0.4 (0.2)	0.4 (0.4)
0.05	3.3 (67.7)	1.7 (19.7)	1.2 (16.2)	0.8 (12.2)	0.4 (0.4)	0.4 (1.2)	0.3 (0)	0.3 (0)

^aThe percentages of good spots (SNR ≥ 2 without saturation) are indicated in parentheses. The optimal amounts and concentrations of reference probe and target are indicated in boldface.