TABLE 2.
Reference probe amt (%) | Reference target concn (fmol/μl) |
|||||||
---|---|---|---|---|---|---|---|---|
12.5 | 1.25 | 0.625 | 0.25 | 0.125 | 0.0625 | 0.025 | 0.0125 | |
10.00 | 115.5 (80.1) | 67.2 (99.1) | 52.3 (98.9) | 19.5 (100) | 12.2 (100) | 4.67 (96.4) | 2.16 (39.1) | 0.9 (6.6) |
5.00 | 97.0 (91.4) | 52.3 (100) | 36.5 (100) | 15.5 (100) | 5.9 (97.8) | 4.7 (91.8) | 1.1 (9.5) | 0.8 (7.3) |
2.50 | 64.8 (97.8) | 36.1 (100) | 28.8 (100) | 12.1 (100) | 6.9 (98.1) | 3.1 (71.0) | 1.5 (21.5) | 0.7 (18.9) |
1.00 | 35.6 (100) | 18.1 (100) | 15.4 (100) | 6.8 (94.4) | 2.6 (39.0) | 2.0 (35.8) | 0.7 (4.7) | 0.5 (2.0) |
0.50 | 23.9 (100) | 13.8 (100) | 8.7 (99.4) | 4.9 (83.0) | 2.1 (6.2) | 1.3 (14.5) | 0.6 (3.1) | 0.5 (0.4) |
0.25 | 13.8 (100) | 7.6 (95.0) | 4.9 (89.7) | 2.7 (44.0) | 1.1 (9.7) | 0.9 (6.8) | 0.4 (0) | 0.4 (0.2) |
0.10 | 6.2 (91.2) | 3.4 (54.7) | 2.3 (40.3) | 1.3 (15.4) | 0.7 (3.8) | 0.6 (3.1) | 0.4 (0.2) | 0.4 (0.4) |
0.05 | 3.3 (67.7) | 1.7 (19.7) | 1.2 (16.2) | 0.8 (12.2) | 0.4 (0.4) | 0.4 (1.2) | 0.3 (0) | 0.3 (0) |
The percentages of good spots (SNR ≥ 2 without saturation) are indicated in parentheses. The optimal amounts and concentrations of reference probe and target are indicated in boldface.