TABLE 1.

Activities of enzymes at the end of the aerobic culture phase and 12 h after the onset of the anaerobic phase

Fermentation typea	Stageb	Mean sp act of enzyme ± SD (U/mg protein)c
		PCK	PPC	MDH	PYK	ICL
I	Aerobic	0.82 ± 0.05	0.22 ± 0.05	21.97 ± 0.15	1,175 ± 11.38	0.12 ± 0.00
	Anaerobic	0.55 ± 0.02	0.19 ± 0.00	18.27 ± 1.05	978 ± 12.33	0.09 ± 0.00
II	Aerobic	1.46 ± 0.10	0.23 ± 0.04	25.69 ± 0.37	2,053 ± 3.65	0.73 ± 0.03
	Anaerobic	1.09 ± 0.01	0.20 ± 0.01	35.55 ± 0.78	1,430 ± 13.78	0.41 ± 0.02
III	Aerobic	2.38 ± 0.11	0.16 ± 0.00	23.5 ± 0.13	2,955 ± 8.77	1.37 ± 0.00
	Anaerobic	1.75 ± 0.03	0.21 ± 0.01	43.8 ± 0.62	2,501 ± 10.15	1.02 ± 0.01

^aFermentation types were mentioned in culture conditions section.

^b“Aerobic” represents the data obtained at the end of aerobic culture; “Anaerobic” represents those obtained 12 h after transition to anaerobic fermentation.

^cThe standard deviations (SD) were calculated from triplicate samples of the same run.