FIG. 2.
Construction of T. kodakarensis TS521 and TS517. In this figure, as well as in Fig. S2 in the supplemental material and Fig. 4, the gene(s) targeted for deletion are identified by a bold outline. (A) Plasmid pTS521 was constructed by cloning the genes, as shown, into pUC118 and used to transform T. kodakarensis KW128. A representative transformant, designated T. kodakarensis TS521, selected by growth on plates lacking tryptophan, had the genome structure shown. (B) Plasmid pTS517 was constructed by cloning the genes, as shown, into pUC118 and used to transform T. kodakarensis KW128. A representative transformant, designated T. kodakarensis TS517i, was selected by growth on plates lacking tryptophan and had the genome structure shown. Dilutions of a culture of T. kodakarensis TS517i were spread on plates containing 6MP, and a 6MPr tryptophan auxotroph, designated T. kodakarensis TS517, had the genome structure shown.