FIG. 3.

Assays of β-glycosidase activity in T. kodakarensis cell lysates. Cells were harvested from cultures of the different T. kodakarensis strains (see Table 1) grown in ASW-YT-S° medium to an OD₆₀₀ of ∼0.6. The cells were lysed, and aliquots of the cleared lysates were incubated with ONP-manno (light gray bars) or ONP-gluco (dark gray bars) for 10 min at 85°C. The release of ONP was measured by the increase in A₄₀₅. The results shown above each strain designation are the averages (with error bars) of triplicate assays of lysates from three separate cultures of that strain. The open bars show the results of assays with aliquots of T. kodakarensis KW128, TS372, and TS1079 lysates that were autoclaved for 20 min at 121°C before incubation with ONP-gluco.