Effect of EMA on amplification of C. jejuni DNA from bovine feces using a Campylobacter genus primer set. The Campylobacter genus amplicon is 816 bp in size, and the IAC is 465 bp in size. Lane 1, 100-bp molecular weight marker; lane 2, feces inoculated with viable C. jejuni cells; lane 3, feces inoculated with viable cells treated with EMA; lane 4, feces inoculated with heat-killed cells; lane 5, feces inoculated with heat-killed cells treated with EMA (note the IAC control amplicon); lane 6, uninoculated feces; lane 7, uninoculated feces treated with EMA. No PCR products were observed in the negative control reaction (not shown).