TABLE 3.
Day | PCR result for non-EMA-treated compost by locationa |
PCR result for EMA-treated compost by locationa |
||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Replicate 1 |
Replicate 2 |
Replicate 1 |
Replicate 2 |
|||||||||||||
Top | Center | Top | Center | Top | Center | Top | Center | |||||||||
0 | − | − | − | − | − | + | − | − | I | + | + | I | + | + | I | + |
7 | − | − | + | − | − | − | − | − | + | + | + | I | + | + | + | + |
15 | + | + | I | + | + | I | + | I | + | I | + | + | + | + | + | + |
21 | + | I | + | + | − | + | + | I | + | + | + | + | + | + | + | + |
36 | + | + | − | + | + | + | + | + | + | + | + | + | + | + | + | + |
49 | + | + | + | + | − | + | + | + | + | + | + | + | + | + | + | + |
63 | + | + | + | + | + | + | + | + | I | + | + | + | + | + | + | + |
77 | + | I | − | + | − | + | + | + | + | + | + | + | I | I | + | + |
98 | + | + | − | I | I | + | + | + | + | I | + | + | + | + | + | + |
126 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | I |
231 | + | + | + | + | − | − | + | + | I | − | + | + | + | + | + | + |
Total positive (%) | 80.0 | 70.0 | 60.0 | 80.0 | 94.1 | 100.0 | 100.0 | 100.0 |
Extracted DNA was subjected to Campylobacter genus-specific PCR. Each replicate compost pile was oriented east to west. For each day and location, the first result represents the east sample location and the second result represents the west sample location. Results were considered inconclusive (I) if neither a genus nor an IAC amplicon was detected.