Figure 2.

A schematic representation of ANGPTL4 coding region. The locations of variant sites are colored according to their affect on protein functionality as previously described [4]. Yellow sites prevent protein secretion, orange sites cause a non-functional protein to be secreted, and black sites were not tested in vitro. Amino acid sites identified by PolyPhen as “possibly damaging” are indicated in light red; “probably damaging” sites are shown in dark red. Radically changing (categories 6, 7, and 8) amino acid sites identified by TreeSAAP are shown in blue, with category 8 sites in dark blue.