Figure 12. Activation of antiparasitic function by exudate fluid.

Wild-type (WT) or IFNγR1^−/−PEM (1 × 10⁵) were cultured for 1d in adherent 96-well dishes and then treated overnight with medium containing either 100 U/ml IFNγ, 20% cell-free exudate fluid prepared from infected mice (Exudate fluid), or no addition (Medium). Cells were then infected for 1d with GFP-RH at moi of either 0.1 or 2, in the presence or absence of the nitric oxide synthase inhibitor L-NAME (2.5 mg/ml). Data from the two infection levels were similar and were pooled for analysis. Cells were harvested by treatment with Accutase for 10 min and assessed for parasite content by flow cytometry, using fluorescent beads to normalize sample volume (n = 4). * p < 0.05. The data are representative of two experiments.