Skip to main content
. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Immunol. 2009 Oct 21;183(10):6667. doi: 10.4049/jimmunol.0900516

Figure 3. Cell type distribution of egressed parasites after reinvasion in vivo.

Figure 3

Adoptive transfer was performed as in Fig. 2, using two donors (one each infected with YFP-RH or GFP-RH) and a total of five host mice. Samples were collected either before transfer (to determine donor cells and donor burden), 1 h post-transfer (to determine host cells and host burden), or 3 h post-transfer (to determine ‘non donor-associated donor parasite’ burden [NDAP]). The proportion of macrophages (Mφ), B and T cells was assessed for donor and host cells by flow cytometry after staining with antibodies against F4/80, B220 or Thy1. For each donor cell type, the fraction of total donor parasite residing in that population was determined (% donor burden), and, similarly, the fraction of total host parasite residing in each host cell type (% host burden). These distributions were then compared to the fraction of NDAP residing in each cell population. The data are representative of two experiments for lymphocytes and four experiments for macrophages.