Figure 7. Role of exudate cell types in ETE.

Exudate cells were prepared from mice infected for 5d with wild-type RH and depleted of specific cell types. In experiment 1 (A - E), exudates were depleted of either 1A8+ cells (neutrophils), the combination of 1A8+ cells, B220+ cells and Thy1+ cells (neutrophil/B cell/T cell triple depletion), or F4/80+ cells (macrophages), or else mock-depleted. (A, B) Dot plots of cells triply stained with 1A8-PE/B220-APC/Thy1-APC antibodies, before (A) or after (B) depletion. Histograms of cells stained with F4/80 before (C) or after (D) depletion illustrate the partial depletion of macrophages. In experiment 2 (F, G), exudates were depleted of either 1A8+ cells or the combination of CD11b+ cells and F4/80+ cells (neutrophil/macrophage double depletion). (F) Histogram of exudate cells stained with F4/80 before (grey) or after (black) double depletion of neutrophils and macrophages. (E, G) Donor PEM infected overnight with YFP-RH (moi = 1) were labeled with DDAO-SE and cultured for 1 h in non-adherent dishes with either medium (None) or a 5-fold excess of exudate cells depleted as indicated. NDAP (as percent of total donor parasites) was determined before (grey bars) and after (black bars) co-culture. Bars represent mean ± S.E. (n = 3). * p < 0.05. ** p < 0.01 (relative to mock depletion).